The study conducted by Bruno Kovač and colleagues underscores the effectiveness of benzalkonium chloride (BAC) as an active compound against foodborne pathogens biofilm. BAC, a chlorine-based chemical compound, exhibits antimicrobial properties against bacteria, viruses, and fungi, making it a versatile biocide, cationic surfactant, and phase transfer agent. In the research, ceramic tiles were utilized as a surface for bacterial adherence and biofilm formation.

Following disinfection treatment with 5% and 20% working solutions of BAC, a significant reduction in total biomass was observed for all tested bacteria compared to the control group. This highlights the potent antimicrobial efficacy of BAC in combating biofilm formation.

The findings of this study provide valuable insights into the potential application of BAC, particularly in Aquavet-12, for disinfection and microbial control in various settings, including food processing facilities, healthcare environments, and agricultural operations. By harnessing the antimicrobial properties of BAC, Aquavet-12 offer a promising solution for ensuring hygiene and safety in critical industries.